Anti-thyroid hormone (T4) recombinant antibody or antigen binding fragment
Data di pubblicazione
Data di priorità
Nazionale (domanda nazionale Finlandese), regionale (EP), internazionale (PCT), Fasi Nazionali (Cina, US)
Politecnico di Milano, VTT Technical Research Centre of Finland Ltd
DIPARTIMENTO DI CHIMICA, MATERIALI E INGEGNERIA CHIMICA "GIULIO NATTA"
METRANGOLO PIERANGELO, NEVANEN TARJA, AROLA HENRI, TULLILA ANTTI, ROUVINEN JUHA, PARKKINEN TARJA
The present invention relates to the fields of antibodies, antigen binding fragments and immunodiagnostics. Specifically, the invention relates to a recombinant anti- body or antigen binding fragment for binding thyroid hormone T4 (thyroxine) and halogenated bisphenol A. Also, the present invention relates to an isolated nucleic acid molecule comprising a nucleotide sequence that encodes the recombinant antibody or antigen binding fragment of the present invention, as well as an ex- pression vector and host cell comprising the nucleic acid molecule of the present invention. Still, the present invention relates to a method of producing a recombi- nant antibody or antigen binding fragment for binding T4 thyroid hormone and hal- ogenated bisphenol A, a test kit and an immunoassay comprising the recombinant antibody or antigen binding fragment of the present invention, and a method for determining T4 thyroid hormone and/or halogenated bisphenol A levels in a sam- ple of a subject. Still further, the present invention relates to a method of treating a sample, e.g. an immunoaffinity-based sample preparation method for enrich- ment of the halogenated bisphenol A from a sample.