Methods, devices and systems for microfluidic stress emulation
Data di pubblicazione21-10-2021
Data di priorità03-03-2016
FaseEP, PCT, US
TitolarePolitecnico di Milano, The University of Arizona, The Research Fundation for the State University of New York
DipartimentoDepartment of Electronics, Information and Bioengineering
AutoriAlberto Cesare Luigi Redaelli, Marco Rasponi, Marvin J. Slepian, Danny Bluestein
The invention consists in a method, a device and a system for monitoring thrombogenic state in a subject. The method consists in:
- modeling the shear stress profile of a cardiovascular pathology or a mechanical circulatory support device,
- reproducing the shear stress profile of the pathology or device in a microfluidic device,
- flowing a sample of platelets (separated from blood obtained from the subject) through the microfluidic device,
- testing the platelets activation state of the subject to monitor the thrombogenic state.
The microfluidic device for imparting shear stress on a fluid sample that reproduces the one imparted on the fluid sample by macrofluidic device or pathology. The device comprises a microfluidic channel with narrowing regions to impart shear stress on the fluid sample flowing through the channel (according to a shear stress history probability density function describing the macrofluidic device), a platelet-separating component, a platelet-activation state assay component and a detector.
Moreover, is also disclosed a system for treating a patient having a cardiovascular disease, comprising i) a macrofluidic device that contacts the cardiovascular system of the patient; and a microfluidic device that reproduces the shear stress imparted on blood by macrofluidic device.