Methods, devices and systems for microfluidic stress emulation

Data di pubblicazione

21-10-2021

Codice

DEIB.17.023.A

Stato

Disponibile

Data di priorità

03-03-2016

Fase

EP, PCT, US

Titolare

Politecnico di Milano, The University of Arizona, The Research Fundation for the State University of New York

Dipartimento

Department of Electronics, Information and Bioengineering

Autori

Alberto Cesare Luigi Redaelli, Marco Rasponi, Marvin J. Slepian, Danny Bluestein

Descrizione

The invention consists in a method, a device and a system for monitoring thrombogenic state in a subject. The method consists in:

• modeling the shear stress profile of a cardiovascular pathology or a mechanical circulatory support device,
• reproducing the shear stress profile of the pathology or device in a microfluidic device,
• flowing a sample of platelets (separated from blood obtained from the subject) through the microfluidic device,
• testing the platelets activation state of the subject to monitor the thrombogenic state.

The microfluidic device for imparting shear stress on a fluid sample that reproduces the one imparted on the fluid sample by macrofluidic device or pathology. The device comprises a microfluidic channel with narrowing regions to impart shear stress on the fluid sample flowing through the channel (according to a shear stress history probability density function describing the macrofluidic device), a platelet-separating component, a platelet-activation state assay component and a detector.

Moreover, is also disclosed a system for treating a patient having a cardiovascular disease, comprising i) a macrofluidic device that contacts the cardiovascular system of the patient; and a microfluidic device that reproduces the shear stress imparted on blood by macrofluidic device.

Campo di applicazione

<p> This device could be an optimal candidate for Point of Care, so the analysis performed right next to where the patient is located. </p>

Vantaggi

<p> The proposed microfluidic device translates to the microscale the Device Thrombogenic Emulation approach (DTE approach) developed by Bluestein and colleagues, which evaluate the activation of isolated platelets by fluorescence inhibiting platelet positive feedbacks and aggregation (i.e. coagulation) thus obtaining a linear relation between the agonist activity and the platelet response. In comparison with the standard DTE approach, the microfluidic device does not require the intervention of an expert professional, can be automated and its production costs are extremely low. </p>

Stadio di sviluppo

Conceptual stage completed. The inventors have developed some prototypes that are now performing tests.

Contatto

licensing.tto@polimi.it