“Microatlas”: a miniaturized imaging window for intravital nonlinear microscopy
Data di pubblicazione
28-09-2021Codice
CH.17.099.AStato
DisponibileData di priorità
21-12-2017Fase
International PCTTitolare
Politecnico di Milano - Università degli studi di Milano Bicocca - Consiglio Nazionale delle RicercheDipartimento
Department of Chemistry Materials and Chemical EngineeringAutori
Manuela Teresa Raimondi, Giulio Cerullo, Claudio Conci, Tommaso Zandrini, Roberto Osellame, Giuseppe ChiricoDescrizione
Microatlas is a miniaturized low invasive imaging window for intravital nonlinear microscopy in living organism; A patent application was filed by Politecnico di Milano with the collaboration of Università degli Studi di Milano Bicocca and CNR. This platform allows to quantify an occurring reaction to exogenous factors such as biomaterial, tumor or drug implantation in vivo, drastically reducing employed animals for in vivo experimentations and related costs. This innovative device contains miniaturized scaffolds which guides tissue regeneration of the hosting living organism, and microscopic reference geometries which both allow repositioning the two-photon microscope field of view in different time points and real-time adaptive-optic techniques. The Microatlas has been largely validated into the chicken embryo model to be paired with brand-new biomaterials which require in vivo pre-clinical validation processes. The platform guarantees high level of feature precision thanks to the two-photon laser writing fabrication process. Microatlas platform can be incorporated into complex system that can be industrialized and commercialized.