“Microatlas”: a miniaturized imaging window for intravital nonlinear microscopy
Data di pubblicazione28-09-2021
Data di priorità21-12-2017
TitolarePolitecnico di Milano - Università degli studi di Milano Bicocca - Consiglio Nazionale delle Ricerche
DipartimentoDepartment of Chemistry Materials and Chemical Engineering
AutoriManuela Teresa Raimondi, Giulio Cerullo, Claudio Conci, Tommaso Zandrini, Roberto Osellame, Giuseppe Chirico
Microatlas is a miniaturized low invasive imaging window for intravital nonlinear microscopy in living organism; A patent application was filed by Politecnico di Milano with the collaboration of Università degli Studi di Milano Bicocca and CNR. This platform allows to quantify an occurring reaction to exogenous factors such as biomaterial, tumor or drug implantation in vivo, drastically reducing employed animals for in vivo experimentations and related costs. This innovative device contains miniaturized scaffolds which guides tissue regeneration of the hosting living organism, and microscopic reference geometries which both allow repositioning the two-photon microscope field of view in different time points and real-time adaptive-optic techniques. The Microatlas has been largely validated into the chicken embryo model to be paired with brand-new biomaterials which require in vivo pre-clinical validation processes. The platform guarantees high level of feature precision thanks to the two-photon laser writing fabrication process. Microatlas platform can be incorporated into complex system that can be industrialized and commercialized.