Selective Plane Illumination Microscopy on a chip

Data di pubblicazione

12-10-2015

Codice

FI.15.024.A

Data di priorità

28-09-2015

Fase

Patent application in Europe and US

Titolare

Politecnico di Milano e Consiglio Nazionale delle Ricerche (CNR)

Dipartimento

Department of Physics

Autori

Andrea Bassi, Petra Paiè, Roberto Osellame, Francesca Bragheri

Descrizione

A millimeter-scale optofluidic lab-on-chip integrates selective plane illumination and continuous sample delivery in a microfluidic channel. The device, fabricated by femtosecond laser micromachining, includes one or more optofluidic cylindrical lenses with an engineered profile to create an aberration-free light-sheet illumination across a fluidic channel. Biological samples are circulated by a microfluidic pump and scanned under a wide field microscope. No alignment from the end-user is required, enabling fast and automatic three-dimensional SPIM scanning.

Campo di applicazione

Optical Microscopy; Biology.

Vantaggi

• Compact and portable: can be used as add-on in commercial upright or inverted microscopes without the need of any motorized stage. • Versatile fluidic design: can image samples at different spatial scales, from single cells to organoids or embryos. • Versatile optical design: suitable for multiple sided illumination, multicolor excitation and double sided detection. • High-throughput: high acquisition rate (> 1 sample/s demonstrated on intact 250 µm diameter cellular spheroids and 100 frames/sample). • Modular: easy integration of multiple lab-on-chip functionalities.

Stadio di sviluppo

Working Prototype

Contatto

licensing.tto@polimi.it