Selective Plane Illumination Microscopy on a chip
Data di pubblicazione
Data di priorità
Patent application in Europe and US
Politecnico di Milano e Consiglio Nazionale delle Ricerche (CNR)
Department of Physics
Andrea Bassi, Petra Paiè, Roberto Osellame, Francesca Bragheri
A millimeter-scale optofluidic lab-on-chip integrates selective plane illumination and continuous sample delivery in a microfluidic channel. The device, fabricated by femtosecond laser micromachining, includes one or more optofluidic cylindrical lenses with an engineered profile to create an aberration-free light-sheet illumination across a fluidic channel. Biological samples are circulated by a microfluidic pump and scanned under a wide field microscope. No alignment from the end-user is required, enabling fast and automatic three-dimensional SPIM scanning.
Campo di applicazione
• Compact and portable: can be used as add-on in commercial upright or inverted microscopes without the need of any motorized stage.
• Versatile fluidic design: can image samples at different spatial scales, from single cells to organoids or embryos.
• Versatile optical design: suitable for multiple sided illumination, multicolor excitation and double sided detection.
• High-throughput: high acquisition rate (> 1 sample/s demonstrated on intact 250 µm diameter cellular spheroids and 100 frames/sample).
• Modular: easy integration of multiple lab-on-chip functionalities.
Stadio di sviluppo